Scientist I (Gene Therapy/ Cell Cloning)

Our client, a world
leader in life sciences and biotechnology, is looking for a “Scientist I” based out of San Diego, CA.

Job Duration: Long
Term Contract (Possibility Of Extension)

Pay Rate : $46.43/hr on
W2

Company
Benefits: Medical, Dental, Vision, Paid Sick leave, 401K

The Scientist I will be
a vital contributor to our non-viral cell and gene therapy delivery platform,
primarily focused on R&D for innovative nucleic acid payload technologies.
This role involves evaluating novel mRNA and DNA designs using a range of
molecular biology techniques, including plasmid cloning, in vitro validation,
and the production of in vitro-transcribed mRNA. To verify successful
expression, the scientist will utilize assays such as Western blot, qRT-PCR,
and luciferase reporter assays.

Job Responsibilities:

·       Design and clone
expression vectors and IVT donor plasmids to support research initiatives.

·       Execute DNA and mRNA
transfections in mouse and human cell lines.

·       Assess nucleic acid
expression through molecular methods like qPCR, reporter assays and Western
blotting.

·       Maintenance of detailed
electronic laboratory notebooks (ELN) and preparation of summary slides

Required Qualifications:

• Bachelor's
  degree with 5+ years or a Master's degree with 2+ years of experience in
  molecular biology, pharmaceutical sciences, or a related field.
• Molecular
  Biology: Required
  proficiency in advanced cloning (Gibson assembly, Golden Gate,
  site-directed mutagenesis) and IVT.
• Functional
  Assays: Hands-on
  expertise in measuring transgene delivery success via reporter assays and
  qRT-PCR.
• Cell
  Culture: Preferred
  experience in genetic manipulation of cell lines and primary cells (e.g.,
  T cells, monocytes, hepatocytes).
• Desired
  proficiency in Snapgene, Prism, and MS Office.

If interested, please
send us your updated resume at

[email protected]/[email protected]